Simultaneous Determination of Oleanolic Acid and Ursolic Acid in Hedyotis Diffusa with HPLC / 中国药房

ABSTRACT

OBJECTIVE:To develop a RP-HPLC method for simultaneous determination of oleanolic acid and ursolic acid in Hedyotis diffusa.METHODS:The optimum HPLC conditions were as follows:Nucledure C 18 column(4.6mm?150mm);mobile phase:acetonitrile-potassium phosphate,pH=7.84(45∶55);detection wavelength:220nm.RESULTS:The average recoveries of oleanolic acid and ursolic acid were(96.79?2.42)%and(97.15?2.33)%respectively.CONCLUSION:The met_ hod could detect oleanolic acid and ursolic acid in Hedyotis diffusa simultaneously.It could be used in the quality control of the herb.