Simultaneous Determination of Phenobarbital, Phenytoin and Carbamazepine in Serum by RP - HPLC / 中国药房
China Pharmacy
;
(12)1991.
Article
in Chinese
| WPRIM
| ID: wpr-522477
ABSTRACT
OBJECTIVE:
To establish a RP - HPLC method for simultaneous determination of the concentrations of phe-nobarbital, phenytoin and carbamazepine in serum.METHODS:
Phenobarbital, phenytoin and carbamazepine were extracted from serum with CH2Cl2 and analysed using C18 column with mobile phase consisting of methanol - water(57 43); the column temperature was 30℃ ; the detecting wavelength was 254nm and the flow rate was 0.8ml/ min.RESULTS:
The calibrating curves of phanobarbital, phenytoin and carbamazepine were linear in the ranges of 2.5 - 40, 2.5-40 and 1.25-20?g/ ml respectively .The within- day and between- day RSDs were less than 10% (n = 5) .CONCLUSION:
This method is simple and reliable and suitable for phenobarbital,phanytoin and carbamazepine monitoring.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
1991
Type:
Article
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