Construction and sequence analysis of eukaryotic expression vector of Chinese prostate-specific membrane antigen / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1989.
Article
in Chinese
| WPRIM
| ID: wpr-522774
ABSTRACT
AIM:
To obtain eukaryotic expression vector of Chinese prostate-specific membrane antigen.METHODS:
Chinese prostate-specific membrane antigen (PSMA) cDNA was amplified by RT-PCR from prostate cancer tissues, then cloned into eukaryotic expression vector pcDNA3 0 and sequenced.RESULTS:
Seven bases in Chinese PSMA cDNA sequence were found different from those reported by Israeli, which lead to two different amino acids.CONCLUSION:
We have obtained the PSMA cDNA, and the recombinant eukaryotic expression vector was successfully constructed. The study lays foundation for DCs vaccine modified by PSMA gene for the treatment of prostate neoplasms.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1989
Type:
Article
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