Effects of fructose-1,6-diphosphate on adriamycin-induced calcium and sarcoplosnic reticulum Ca ~(2+)-ATPase activity in cardiomyocytes of rats / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1989.
Article
in Chinese
| WPRIM
| ID: wpr-522789
ABSTRACT
AIM:
To study the effects of fructose-1,6-diphosphate (FDP) on adriamycin (ADR)-induced calcium and sarcoplasmic reticulum Ca 2+-ATPase activity in cardiomyocytes of rats.METHODS:
Rats were treated with ADR by intraperitoneal injection (2 5 mg?kg -1 body weight) once every two days for 11 days, and then ADR-treated rats were intervened by FDP at different dosages (ip) once every other day for 41 days. Enzyme linked immune absorption assay (ELISA) was employed to detect froponin I (CTnI). CK-MB was examined by monoclonal antibody. Intracellular free calcium concentration was measured on fluorescent spectrophotometry and SRCa 2+-ATPase activity was examined by inorganic phosphate.RESULTS:
FDP (300, 600, 1 200 mg?kg -1) significantly reduced the levels of CTnI and CK-MB in serum. Decreased calcium and increased SRCa 2+-ATPase activity in cardiomyocytes were also observed when ADR-treated rats were intervened by FDP (P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1989
Type:
Article
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