Quantitative Determination of Troxerutin Injection by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To determine the content of Troxerutin in Troxerutin injection by HPLC METHODS:The separation was performed on a Hypersil BDS C18 column The mobile phase was composed of acetontrile-tetrahydrofuran-0.05 mol/Lsodium dihydrogen phosphate(15∶8∶77V/V ) ,adjusted to pH4 0 with phosphoric acid The flow rate was 0 8ml/ min The ultraviolet wavelength was 254nm RESULTS:The calibration curve showed good li nearityinrange of (20～200) ?g/ ml(r=0 9998) ;The mean recovery was 100 7 % The within -day RSD was 1 61 %,and inter-day RSD was 2 46 % CONC_ LUSIONThe method was simple ,convenient and accurate and can be used for the quality control of Troxerutin injection