Determination of Cefixime in Human Plasma with HPLC / 中国药房
China Pharmacy
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-523206
ABSTRACT
OBJECTIVE:
To establish a HPLC method for determining cefixime in human plasma.METHODS:
Analytical column was Discovery C 18 (4.6mm?250mm,5?m),the mobile phase consisted of methanol-acetate buffer-triethylaming(28∶72∶0.5V/V),the flow rate was1.0ml/min.The detection was performed at UV286nm.Plasma concentrations of cefixime and internal standard(Cephradine)were determined by HPLC.RESULTS:
The calibration curve was linear in the concentrations ranging from0.1to5.0?g/ml(r=0.9995).The detection limit was0.1?g/ml.The recovery was(96.63?3.17)%.The within-day RSD was less than6.10%and between-day RSD was less than6.69%.CONCLUSION:
The method is simple,sensitive,accurate and suitable for determination of cefixime in human plasma and pharmacokinetic study.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2001
Type:
Article
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