Determination of Icariin in Ruzenning Tablets by Column Chromatography-HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a method for determining the content of icariin in Ruzenning tablets.METHODS:Column chromatography-HPLC was adopted.C 18 column was used with acetonitrile-water(30∶70)as mobile phase and the detection wavelength was set at270nm.RESULTS:The linear range was10?g～18?g for the icariin(Y=0.9995).The average recovery was94.6%,RSD=1.95%(n=6).CONCLUSION:The method is simple and accurate with a good reproducibility and can be used as a quantitative analysis method for this preparation.