Effects of angiotensin II and losartan on collagen synthesis in rat hepatic stellate cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-523507
ABSTRACT
AIM:
To investigate the effects of angiotensin II (Ang II) and AT_(1a) receptor antagonist (losartan) collagen synthesis in rat hepatic stellate cells (HSCs).METHODS:
① Rat HSCs were isolated, cultured and identified. ② Rat HSCs were incubated in the medium with different concentrations of AngII or losartan, then the quantity of collagen was examined by [~3H]-proline release assay.RESULTS:
① The yield of HSCs was 2?10~7-(3?10~7/per) rat, their viability and purity was more than 95% and 90%, respectively. ② The yield of collagen in HSCs significantly got a rise in a concentration-dependent manner when HSCs were incubated with AngII (10~(-6)mol/L-10~(-10) mol/L) (P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2000
Type:
Article
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