Determination of Triptolide and Triptonide in plasma by HPLC / 中国法医学杂志
Chinese Journal of Forensic Medicine
; (6)2002.
Article
in Zh
| WPRIM
| ID: wpr-523541
Responsible library:
WPRO
ABSTRACT
Objective To establish a method for quantitative determination of triptolide and triptonide in human plasma samples by HPLC.Methods The samples were extracted with Oasis HLB solid phase extraction cartridge and analyzed with reserved phase HPLC coupled with diode array detection.Results Recoveries rates obtained from spiked plasma for the two diterpenoid were higher than 80%.The linearity ranged from 10 ng/ml to 1 000 ng/ml.Detection limits of the method were 3.0ng/ml for triptolide and 4.5ng/ml for triptonide.Conclusion The method is fast and accurate,which is applicable to determination of diterpenoid in cases of suspected poisoning of Triptergium wilfordii Hook.f. in forensic practice.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Forensic Medicine
Year:
2002
Type:
Article