Content Determination of Paeoniflorin in Weile Capsule with HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish HPLC method for determination of the content of paeoniflorin in Weile capsules.M_ ETHODSPaeoniflorin was separated on Hypersil C 18 column by using acetonitrile-0.1%phosphoric acid(18∶32)as mobile phase and230nm as UV detection wavelength.RESULTS:There was a good linear relationship between the concentration of paeoniflorin and absorption area value in range of0.25?g～2.5?g.The average recovery was97.9%(RSD=1.09%,n=5). CONCLUSION:The method is highly reproducible and accurate.