Effect of hypoxia on expression and function of integrin rece ptor ?_v?_3 of bovine retinal v ascular endotheliocytes / 中华眼底病杂志

ABSTRACT

Objective To investigate the effect of hypoxia on the e xp ression and function of integrin receptor ? v? 3 of bovine retinal vascular endotheliocytes. Methods Bovine retinal vascular endotheliocy tes in the culture dishes coated by vitronectin was put into the normal and hypo xemic condition, respectively. Enzyme linked immunosorbent assay and cel l-adhesion analysis were used to detect the expression and function of integrin receptor ? v? 3 in bovine retinal vascular endotheliocytes, respectively. Results Under the condition of hypoxia, the expression of ? v? 3 increased gradually, and reached the peak at the 48th hour. The expressio n of ? v? 3 at the 60th and 72nd hour in hypoxia group was higher than that in the normal group. Bovine retinal vascular endotheliocytes absorbed more Vn of extra-cellular matrixes (ECM) after cultured under hypoxemic condition for 24 hours. Conclusion Hypoxia may up-regulate the expression of ? v? 3, which promote the adsorbability of endotheliocytes.