Changes of nitric oxide synthase in lung of rats after ischemic reperfusion of hind limbs / 中国病理生理杂志

ABSTRACT

AIM: To study the distribution of nitric oxide synthase (NOS) in normal lung of rats, and the changes in distribution and activity of NOS in lung of rats suffering from limb ischemia reperfusion (LIR). METHODS: Using tourniquet, the model rats undergoing ischemia reperfusion were made. Using ?-ANDPH-d histochemical method, computer analysis of image and spectrophotography, the distribution of NOS in normal lungs of rats, and the changes in distributon and activity of NOS in lung of rats suffering from limb ischemia reperfusion (LIR) were observed. RESULTS: Histochemically, in normal rats, the NOS positive staining was found in epithelial cells of respiratory duct including bronchi, alveolar duct as well as in endothelium of pulmonary vessels, and the negative staining was found in pneumonocytes. In LIR rats, the above NOS staining were significantly stronger than that in normal one, and positive staining was also found in vascular smooth muscle and pneumonocytes. Biochemically, the results showed that the values of lung NOS, NO in LIR group increased significantly compared with the normal control. CONCLUSION: NO may play an important role not only in physiology of lung but also in pathophysiology of acute lung injury following LIR. [