Allele and haplotype frequencies of 10 Y-chromosome STR loci in Han population in Zhuhai area / 中国法医学杂志

ABSTRACT

Objective To investigate allele frequency distributions and haplotype polymorphism of ten Y-chromosome short tandem repeats (STR) loci of Han population in Zhuhai area and evaluate their applicability to forensic cases. Methods DYS393, DYS19, DYS389 Ⅱ, DYS390, DYS391, DYS389 Ⅰ, DYS439、DYS438、DYS392 and DYS385 loci were amplified with Y-plex~(TM) PCR amplification Kit and analyzed by ABI 310 genetic analyzer. 200 unrelated male individuals of Han nationality in Zhuhai were recruited for determination of allele frequencies and haplotype data. Results 5, 6, 6, 5, 4, 5, 5, 5, and 7 alleles were found in 9 of the 10 Y-STR loci, respectively and 44 haplotypes were detected at DYS385 locus. The range of gene diversity (GD) was determined between 0.3904 for DYS391 and 0.9497 for DYS385. Total 161 haplotypes were found at the 10 Y-STR loci, of which 134 occurred once, 20 occurred twice, 3 occurred three times, 3 occurred four times, and 1 occurred five times. The cumulative GD was 0.9948. Conclusion The 10 Y-STR loci are highly polymorphic and suitable for forensic individual identification and paternity testing in Zhuhai Han population.