Potential of human bone marrow mesenchymal stem cells differentiating into hematopoietic cells with fetal liver stromal cell-conditioned medium in vitro / 中国病理生理杂志

ABSTRACT

AIM: To explore the potential of human bone marrow mesenchymal stem cells (hMSCs) differentiating into hematopoietic cells with murine fetal liver stromal cell-conditioned medium (FLSC-CM) in vitro. METHODS: 12.5-14.5 days post coitus (dpc ) of KM mice were used for the preparation of fetal liver stromal cell-conditioned medium (FLSC-CM) and embryonic fibroblast feeder layer (FD). Culture-expanded hMSCs were directly contacted with FLSC-CM, FD, and the combination of human interleukin-6 (IL-6) or stem cell factor (SCF), respectively. Seven days later, the non-adherent cells were collected and characterized by morphology, immunophenotypes, and colony forming unit-granulocyte/macrophage culture assay. RESULTS: The number of nonadherent cells derived from hMSCs cultured with FLSC-CM was increased remarkably than those with either FD or cytokines. The non-adhered cells with the morphology of monocyte-or small lymphocyte-like cells were positive for human CD34, CD45 and had the capacity to form the hematopoietic progenitor colonies in methylcellulose cultures containing recombined human granulocyte/macrophage-colony stimulating factor (rhGM-CSF). CONCLUSION: hMSCs were successfully induced toward their differentiation into CD34+CD45+ hematopoietic progenitors after being cultivated with FLSC-CM. This study suggests that hMSCs have the hematopoietic differentiation potential in vitro. [