The Effect of Bone Marrow Mesenchymal Stem Cells on Graft Versus Host Disease and Survival Rate After Allogeneic Bone Marrow Transplantation / 中国医师杂志

ABSTRACT

Objective To observe the effect of bone marrow mesenchymal stem cells (BMMSC) on graft versus host disease (GVHD) and survival rate after allogenic bone marrow transplantation (allo-BMT) in acute lymphocytic leukemia (ALL) mice. Methods In the experimental group, both bone marrow cells and BMMSC obtained from donor mice were transplanted into the recipient mice injected with leukemia cells five days ago, and in the control group, the mice was injected with bone marrow cells alone. The impact of BMMSC on the quantity of CD 4 + and CD 8 + T cells after allo-BMT was evaluated by flow cytometry. The general manifestation and pathological changes of GVHD were observed. The survival time of recipient mice was recorded. Results BMMSC could decrease the quantity of CD 4 + T cells, increase CD 8 + T cells number, delay GVHD, and obviously increase the survival time in the mice treated with allo-BMT(P