The Effect of ~(60)Co Brachytherapy on the Gene Expession of Fibronectin and MMP-2 / 中国医师杂志

ABSTRACT

Objective To study the effect of 60 Co radiotherapy on the gene expession of fibronectin (FN) and matrix metalloproteinase-2 (MMP-2) in the cultured rat vascular smooth muscle cells (VSMCs). Methods Rat VSMCs were cultured in DMEM containing 10% FBS, and were radiated by 60 Co at the doses of 0,7,14 and 28Gy, respectively. mRNA level of FN and MMP-2 was measured by RT-PCR. Results The expression level of MMP-2 mRNA decreased from 82.52?6.50 to 64.25?3.81 and 42.17?3.16 in VSMCs with 14,25Gy radiation, respectively(P