The Change of CD_4~+CD_(25)~+ and CD_8~+CD_(28)~- T Regulatory Cells in Rats with the Experimental Colitis Induced by TNBS / 中国医师杂志

ABSTRACT

Objective To explore the change and function of CD 4 +CD 25 + and CD 8 +CD 28 -T regulatory cells in peripherel blood monocyte cell (PBMC), spleen and colon in rat experimental colitis induced by trinitrobenzenesulfonic acid (TNBS) . Methods The rat model of experimental colitis was established by enema with TNBS/ethanol. The proportion of CD 4 +CD 25 + and CD 8 +CD 28 - T regulatory cells in PBMC, spleen and colon was determined by flow cytometry at the first and third weeks after establishing model, respectively. Results The model of experimental colitis in rats was successfully established(n=15). Compared with control group, the proportion of CD 8 +CD 28 -T regulatory cells significantly increased in colon at the first week after establishing model [(12.7?5.4)% versus(3.87?3.7)%,P