Effect of photodynamic reaction and sclerosant on venous endothelial cells / 中华消化内镜杂志

ABSTRACT

Objective To observe the effect of photodynamic reaction and sclerosant on venous en dothelial cells and to evaluate the potential significance of photodynamic therapy on esophageal varicose. Methods Endothelial cells of human umbilical vein ( ECV304) were cultured in DMEM medium containing 10% fetal bovine serum; 2. 5, 5, 10 and 20 mg/L of hematoporphyrin monomethyl ether were added to the cell mediums then received 5 or 10 minutes of irradiation by copper vapor laser at power density 15 mW/ cm2. MTT colorimetric assay was used to detect the cell survival rate, then drew the cell growth curve and observed the morphological changes of venous endothelial cell induced by photodynamic and sclerosant. Results Cell survival rate decreased along with the increasing dosage of irradiation or hematoporphyrin monomethyl ether. When hematoporphyrin monomethyl ether was 10 mg/L and irradiation time was 10 minutes or hematoporphyrin monomethyl ether was 20 mg/L and irradiation time was 5 minutes, the cell survival rate was less than 50%. Normal endothelial cells entered exponential growth stage from the 2nd day of culture, showing an "S" shape growth curve. After photodynamic irradiation the endothelial cells grew slowly and decreased from the 2nd day of culture, showing a low flat growth curve without exponential growth stage. Number of cells in photodynamic group was significantly lower than those of control group at 2nd, 3rd, 4th, 5th and 6th day ( P = 0. 002 , P