Role of STAT3 in gut mucosal barrier dysfunction during severe acute pancreatitis / 中国普通外科杂志

ABSTRACT

Objective To determine the correlation between intestinal epithelial cell apoptosis; and STAT3 activation during murine severe acute pancreatitis (SAP).Methods Wistar rats were divided into four groups (12 in each group) S (sham), C (control), A (SAP), N (NAC was given 1 hour before SAP).Samples were obtained 3 or 24 hours after for the determination of plasma D-lactate, intestinal mucosa MDA and XO, immunohistochemical staining for PCNA.Apoptosis of the epithelial cell was examined with TUNEL and DNA laddering.The expressions of Bcl-2? Bax? p-STAT3 in intestinal mucosa were assessed by Western blot.Results Plasma D-lactate and intestinal epithelial cell apoptosis increased in group A, especially at 24 hours after operation ( P