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The Effect of Candida albicans on the Expression Level of Human ?-defensin-2 mRNA in Keratinocytes in vitro / 中华皮肤科杂志
Chinese Journal of Dermatology ; (12)1995.
Article in Chinese | WPRIM | ID: wpr-524503
ABSTRACT
Objective To investigate the effect of Candida albicans and its components on the expression level of human beta-defensin-2 mRNA (HBD-2) in keratinocytes in vitro. Methods Different components of Candida albicans were isolated by lyticase, repeated freezing and thawing, sonication, and centrifugation. The keratinocytes and HaCaT cell lines were co-cultured with Candida albicans and its cellular components for 24 h. The expression level of HBD-2 mRNA was detected by reverse-transcription polymerase chain reaction (RT-PCR). Results Low expression level of HBD-2 mRNA in the unstimulated keratinocytes and HaCaT cells was detected. The HBD-2 mRNA expression levels in the keratinocytes stimulated by Candida albicans, the extract of its cell wall, and pure mannan were significantly increased (P 0.05). Conclusions Candida albicans, the extract of cell wall of Candida albicans, and commercial mannan can increase the expression level of HBD-2 mRNA in keratinocytes.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Dermatology Year: 1995 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Dermatology Year: 1995 Type: Article