Content Determination of Bacterial Endotoxin in Puerarin and Glucose Injection by Kinetic-turbidimetric Technique / 中国药房
China Pharmacy
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-524510
ABSTRACT
OBJECTIVE:
To establish a method for the contents determination of bacterial endotoxin in Puerarin and Glucose injection.METHODS:
The kinetic-turbidimetric technique was adopted to dilute the puerarin glucose injection by2-fold,in which,the contents of bacterial endotoxin was determinated by preliminary interference test and interference test.RESULTS:
Diluting the sample by2-fold could effectively eliminate its interference to tachypleus amebocyte lysate(TAL).The average recovery rate was at the range of50%~200%.CONCLUSLONCompared with the pyrogen rabbit de-termination method and the half-quantity gel method,the kinetic-turbidimetic technique has many merits such as high de-termination sensitivity,short cycle,accurate result and better reproduction quality,etc.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2001
Type:
Article
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