The Construction of bFGF/Math1 Gene Vector and Its Expression in the Cochlea of Rat / 听力学及言语疾病杂志
Journal of Audiology and Speech Pathology
;
(6)1998.
Article
in Chinese
| WPRIM
| ID: wpr-524614
ABSTRACT
Objective To construct the bFGF/Math1 f usion gene expression vector and to investigate its expression in the cochlea of rat. Methods Using the recombinant DNA technique to construct the bFGF/Math1 gene expression vector and the restriction enzyme analysis to ide ntify the correct construction. The mRNA expression was detected by the RT-PCR m ethod after being transfected into the cochlea of rat using lipofectin reagent. Results The recombinant was correct and the bFGF/Math1 wa s expressed in the cochlea of rat.Conclusion The PRK5-bFGF-Math1 eukaryotic expression plas mid was successfully constructed and the bFGF/Math1 was expressed in the mammali an cochlea.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Audiology and Speech Pathology
Year:
1998
Type:
Article
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