Preparation and Content Determination of Collagen Protein Bilayer Minipellet of Recombinant Human Erythropoietin / 中国药房
China Pharmacy
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-524670
ABSTRACT
OBJECTIVE:
To prepare collagen bilayer minipellet of recombinant human erythropoietin(rhEPO)and for which to establish a content determination method.METHODS:
The bilayer minipellet was prepared and shaped by pressuriz-ing gel with the collagen protein as coating material and the sodium chondroitin sulfate as releasing moderator;the content of rhEPO in minipellet was determined by RP-HPLC.RESULTS:
The linear range of rhEPO was0.25~20?g/ml(r=0.9998),the average recovery rate was96.2%,the intraday RSD was0.9%and the interday RSD was2.6%(n=3).CONCLUSION:
The preparation for the rhEPO bilayer minipellet is easy and the content determination method of which is accurate and reli-able.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2001
Type:
Article
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