Role of plk1 in the anti-cancer effect of colcemid and vincri stine against K562 cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1986.
Article
in Chinese
| WPRIM
| ID: wpr-524694
ABSTRACT
AIM:
To study the role of plk1 in the ant i-cancer effect of colcemid and vincristine against K562 cells.METHODS:
K562 cells were treated with colcemid and vincristine a nd antisense oligonucleotide of plk1, then expression of plk1 and ?-tubulin wer e investigated by Western blotting and confocal microscopy.RESULTS:
Treatment of K562 cells with colcemid and vincristine i nfluenced the condensation of plk1 and assembly of ?-tubulin, though without ch ange of protein quantity. Treatment with antisense oligonucleotide of plk1 not o nly reduced the expression of plk1 without influence on protein quantity of ?-t ubulin detected by Western blotting, but also disturbed the formation of centros ome observed by confocal microscopy.CONCLUSION:
The function of colcemid and vincristine destructing the spindle might be realized through the mechanism of restraining condensation of plk1 and assembly of ?-tubulin, which might be dependent on plk1. plk1 may be a potential target in anti-cancer therapy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1986
Type:
Article
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