Effects of angiostatin on the activities of extra-cellular signal-regulated protein kinase in microvascular endothelial cells of rat′s retina / 中华眼底病杂志

ABSTRACT

Objective To observe the effects of angiostatin on the activity of extra-cellular signal-regulated protein kinase (ERK) of retinal microvascular endothelial cells of mice. Methods Angiostatin was separated and purified by l-lysine sepharose 4B from human plasma. The primary retinal microvascular endothelial cells were divided into 4 groups the control group, vascular endothelial growth factor (VEGF) 10 ng/ml group, angiostatin 130 ?g/ml group, and VEGF (10 ng/ml) + angiostatin (130 ?g/ml) group. The expression of ERK-1 was assayed by Western-blotting method 1, 2, 5, 10, 15, and 30 minutes after the treatment of angiostatin. Results Compared with the control group, the expression of ERK-1 reduced 1 minute after treatment, reduced markedly after 10 minutes. After 30 minutes, no differences of the expression of ERK were seen between the control group and angiostatin group. The activation of ERK-1 of retinal microvascular endothelial cells occurred after stimulated by VEGF, and at the pitch at the peak after 5 minutes. The level of ERK in VEGF group increased 210% than that in the control ( P