Construction of human immunoglobulin combinatorial library containing D-Dimer on the surface of phage / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1989.
Article
in Chinese
| WPRIM
| ID: wpr-525143
ABSTRACT
AIM:
To construct a human recombinant immunoglobulin library containing D-Dimer by using phage surface display technology.METHODS:
Human immunoglobulin heavy chain and light chain genes were amplified respectively by RT-PCR from different human lymphocytes using family specific primers and signal sequences of immunoglobulin as half-nested PCR primers. The heavy chain and light chain PCR products were cloned into phagemid vector pComb3H and the human immunoglobulin recombination library was generated with helper phage VCSM13.RESULTS:
A human combinatorial antibody library consisting of 2.8?10~8 in dependent clones was generated with a titer of 4.1?10~(17)PFU/L. The recombinant frequency of Fab genes was 46%.CONCLUSION:
A human combinatorial antibody library was generated. It will be beneficial for selecting Fab antibodies of D dimer from the library.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1989
Type:
Article
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