Effect of promoting cellular cholesterol efflux on the apoptosis of foam cells derived from monocytes / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1989.
Article
in Zh
| WPRIM
| ID: wpr-525176
Responsible library:
WPRO
ABSTRACT
AIM: To determine the effect of promoting cellular cholesterol efflux on the apoptosis of foam cells derived from monocytes. METHODS: RAW264.7 cells were incubated with 50 mg/L ox-LDL as a foam cell mode. The apoptosis rate of RAW264.7 cells was assayed by flow cytometry. Cellular lipid droplet was assayed by oil red staining. The rate of cellular cholesterol efflux was assayed with [~3H] label cholesterol, and the content of cellular cholesterol were assayed by high performance liquid chromatography. RESULTS: After incubation with 50 mg/L ox-LDL for 48 h, the content of cellular cholesterol ester increased from (6.8?3.6) mg/g to (101.7?4.5) mg/g (P
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Pathophysiology
Year:
1989
Type:
Article