The Study of Tumor Suppressor Gene PTEN Expression and Its Correlation with the P53 Expression in Cholangiocarcinoma / 中国医师杂志

ABSTRACT

Objective To investigate the expressions of phosphatase and tensin homolog deleted on chromosome 10(PTEN) and P53 protein in cholangiocarcinoma and evaluate their possible relationship and clinical significance. Methods The expressions of PTEN and P53 in 36 cases of cholangiocarcinoma, eight cases of normal biliary duct and 30 cases of para-neoplastic tissue were detected by S-P immunohistochemistry method. Results The positive rate of PTEN protein expression was 63.89%(23/36) in cholangiocarcinoma, which was significantly lower than that in normal biliary duct (100%,8/8) and that in para-neoplastic tissue(100%,30/30), and which had no relationship with tumor locus, but was significantly correlated with the degree of differentiation and metastasis. The positive rate of P53 protein expression was 44.44%(16/36) in cholangiocarcinoma, which was significantly higher than that in normal biliary duct( 0%, 0/8) and that in para-neoplastic tissue (0%, 0/30), and it had no relationship with tumor locus and metastasis,but was significantly correlated with the degree of differentiation. The PTEN protein expression had correlation with the expression of P53. Conclusion The expression of PTEN protein was significantly lower in cholangiocarcinoma and significantly correlated with the degree of differentiation and metastasis other than the locus of tumor. The expression of P53 protein was associated with the degree of differentiation, but not with the tumor locus and metastasis. Moreover, The PTEN protein expression had correlation with the P53 expression.