Association and function study of tumor necrosis factor receptorⅡ position 196 polymorphism with systemic lupus erythematosus / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1999.
Article
in Chinese
| WPRIM
| ID: wpr-525794
ABSTRACT
AIM:
To investigate the association of gene polymorphism at position 196 of tumor necrosis factor receptor Ⅱ (TNFRⅡ) with systemic lupus erythematosus (SLE) in Chinese, and establish recombinant retroviral vector to analyze the function of the TNFRⅡ 196M/R.METHODS:
The genotype at position 196 of TNFRⅡ was determined by PCR-RFLP in 106 SLE patients and 119 healthy controls in china. Human TNFRⅡ196M cDNA were amplified by PCR and cloned into PMD18-T vector. Then, PMD18-TNFRⅡ196R was induced by site-directed mutagenesis. The recombinant T vector, PMD18-TNFRⅡ196M and PMD18-TNFRⅡ196R, were subcloned into retroviral vector PLXSN. Both normal and variant were transfected into rat mesangial cell. The effects of TNF? on production of sTNFRⅡ and IL-6 were study by ELISA.RESULTS:
(1) The frequency of TNFRⅡ196R allele was significantly higher than those in controls (35.2% vs 14.3%, P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1999
Type:
Article
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