Content Determination of Matrine in It's Liposomes by Acid Dye Colorimetry / 中国药房
China Pharmacy
;
(12)2005.
Article
in Chinese
| WPRIM
| ID: wpr-526188
ABSTRACT
OBJECTIVE:
To establish a method for content determination of matrine in it's liposomes by acid dye colorime_ try.METHODS:
Sephadex gel G-50 column was established by swelling of matrine liposomes sample in distilled water for at least 12 hours,distilled water was used as mobile phase,after eluting,the column was mixed with 0.0 125%of bromothymol bl_ ue buffer solution and chloroform,and then the mixture was shaken,standing and demixed,the absorbability of chloroform layer was detected at the wavelength of 413 nm.RESULTS:
The detection concentration of matrine showed good linearity with its absorbability within the range of 0.04~0.20mg/ml(r=0.9 972),the average recovery was 94.10%(RSD=1.86%).CONCL_ USIONThe established method is simple and accurate,which can be used for quality control of matrine liposomes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2005
Type:
Article
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