Assaying of Polysaccharides in Torreya Yunnanensis and Analysis of HPCE Fingerprints / 中国药房

ABSTRACT

OBJECTIVE:To establish phenol-vitriolic colorimetry for the assaying of polysaccharides in Torreya yunnanensis.METHODS:Detection wavelength was 490nm,slit-width was 2nm and record paper range was 20nm/cm;Torreya yun_nanensis from different origins were analyzed by HPCE.RESULTS:The linear range of anhydrous dextrose was 0.0 114mg～0.1 482mg(r=0.9 994);The average recovery rate was 98.3%(RSD=2.31%,n=5).The HPCE fingerprints of Torreya yu_nnanensis from different origins were basically the same,and 2 kinds of polysaccharides were contained in whcih.CONCLUSI_ONThe contents and compositions of polysaccharides of Torreya yunnanensis from different origins were of no significant diff_erence.