Establishment of two-dimensional gel electrophoresis technical platform for the blood serum proteome research in patients with end stage renal disease / 中国医师杂志
Journal of Chinese Physician
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-526497
ABSTRACT
Objective To establish and optimize the two-dimensional gel electrophoresis technical platform for the blood serum proteome research in patients with end stage renal disease(ESRD).Methods Immobiline pH gradients isoelectric focusing was used as the first dimensional gel electrophoresis and the vertical SDS-PAGE was used as the second dimensional gel electrophoresis(2-DE).The 4 differentially expressed protein spots were identified by mass spectrometry.Results Satisfactory 2-DE maps of ESRD patients serum protein were obtained and there were some differentially protein spots between the 2-DE maps of ESRD patients and normal controls.Conclusions The 2-DE technology for the serum proteome of ESRD patients is set up.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chinese Physician
Year:
2001
Type:
Article
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