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Identification of down-regulated genes in cardiac fibroblasts stimulated by angiotensin Ⅱ / 中国病理生理杂志
Article in Zh | WPRIM | ID: wpr-526560
Responsible library: WPRO
ABSTRACT
AIM: To identify the down-regulated genes in adult rat cardiac fibroblasts (CF) stimulated with angiotensin Ⅱ (AngⅡ). METHODS: Suppression subtractive hybridization (SSH) was performed between the CF stimulated by AngⅡ (driver) and unstimulated CF (tester) to generate subtractive cDNA library. The library was screened with dot blots hybridization to further verify the differentially expressed cDNA clones. Partial positive clones were sequenced and BLAST analyzed. RESULTS: Seventeen down-regulated genes related to intracellular signal transduction, transcriptional repression, deposition of fibrous matrix and cellular cytoskeletal rearrangement, and 4 new expression sequence tags (EST) were acquired. CONCLUSION: SSH is a powerful technique with high sensitivity for the detection and clone of down-regulated genes expressed in CF induced by AngⅡ, which is helpful to clarify the mechanism of cardiac remodeling.
Key words
Full text: 1 Index: WPRIM Type of study: Diagnostic_studies Language: Zh Journal: Chinese Journal of Pathophysiology Year: 1986 Type: Article
Full text: 1 Index: WPRIM Type of study: Diagnostic_studies Language: Zh Journal: Chinese Journal of Pathophysiology Year: 1986 Type: Article