Effect of Radix Isatidis on LPS-stimulated Release of Inflammatory Cell Factors of Mice / 中国药房

ABSTRACT

OBJECTIVE:To investigate the effect of the active site F 022 of Radix Isatidix antiendotoxin on the LPS-stimulated release of inflammatory cell factors in monocytes of mice.METHODS:Monocytes samples taken from the abdominal cavities of BALB/C mice were divided into6groups,the trial group were divided into1%,0.5%,0.25%and0.125%4groups,which added with Radix Isatidis F 022 sample solution and LPS solution;The positive group were treated with LPS solution;The negative group were added with1%F 022 solution.Thereafter the levels of TNF-?,IL-6and NO of the3kinds of inflammatory cell factors cultured in the clear supernatant liquid were determined.RESULTS:LPS could stimulate monocytes of mice to excessively release inflammatory cell factors including TNF-?,IL-6and NO.Compared with the positive group,the levels of inflammatory cell factors in the trial group reduced and which was dose dependent.CONCLU-SIONThe active site F 022 of Radix Isatidix antiendotoxin has inhibitory action on LPS-stimulated excessive release of in-flammatory cell factors of in monocytes of mice.