Inhibition of skeletal muscle proteolysis underlying the treatment of growth hormone combined with parenteral nutrition on surgical critical patients / 中国普通外科杂志

ABSTRACT

Objective To study the effect of growth hormone (GH) combined with parenteral nutrition on skeletal muscle proteolysis for surgical critical patients. Methods Twenty surgical patients with APACHE II scores from 10 to 20 were divided randomly into two groups parenteral nutrition group (PN group,n = 10) ; PN + GH ( GH group,n = 10). At the 5th day and 8th day during the study, urinary output of 3-methylhistidine ( 3-MH ) in 24 h urine were assessed with high performance liquid chromatography; at the 8th day the protein expressions of ubiquitin in quadriceps femoris muscle were determined by immunohistochemistry and the expressions of ubiquitin and C2 mRNA were determined by Northern blot analysis, the ultrastructure of quadriceps femoris muscle was observed under electronic microscope. All data were collected down in the form as mean ? standard deviation and analyzed with SPSS 11.5 for Windows. Results The urine level of 3-MH and the protein, mRNA and C2 mRNA expression of ubiquitin in quadriceps femoris muscle decreased in GH group as compared with PN group ( t = 4. 886, 6. 846,7. 314,5. 588,P