Effect of methylene blue and visible light monitored by flurescence quantitative PCR on the inactivation of plasma hepatitis B virus / 国际检验医学杂志

ABSTRACT

Objective To evaluate the effect of methylene blue and visible light by FQ-PCR assay on the inactivation of plasma hepatitis B virus,and to establish a direct and impersonal basis for the surveillance of cural inactivation.Methods Four samples of plasma of HBV-DNA positive were added with methylene blue. Then the plasma was processed with visible light radiation(30 000 Lux) for 0, 5, 10, 15,30 min respectively. Moreover,the concentration of HBV-DNA samples was quantitatively detected by the method of FQ-PCR and compared with untreated samples to evaluate the effect of HBV inactivation and its relationship with the time of radiation.Results The original concentration of HBV-DNA was 5.6?107, 3.2?106, 1.8?106,3.5?105 copies/ml respectively. After being added with methyene blue, the concentration of HBV-DNA was significantly decreased(P