The function of HGF/SF in the proliferation of colorectal cancer cells / 中国普通外科杂志

ABSTRACT

Objective To study the function of hepatocyte growth factor/scatter factor(HGF/SF) in the (proliferation) of colorectal cancer cells.Methods The expression of c-met,the receptor of HGF,was(detected) in Caco-2 and Colo320 cell lines by Western blot.The activation of p42/p44MAPK and p38MAPK induced by HGF in these two cell lines was observed.Observation of the effect of the inhibitor of p42/p44MAPK(PD98059),p38MAPK(SB203580) on the inhibition of HGF-induced proliferation of Caco-2 and Colo320 cells were made by Using -TdR,MTT assay.Results(1)Both cell lines(expressed) the c-met.(2)HGF activated p42/p44MAPK and p38MAPK,and 20ng/ml HGF treated cells showed maximum activity in both to be within 10min.(p42/p44MAPK,2.28?0.01;p38MAPK,2.25?0.01).(3)HGF was found to significantly increase thymidine incorporation(P