Oridonin enhances phagocytosis of apoptotic U937 cells by macrophage-like cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1986.
Article
in Chinese
| WPRIM
| ID: wpr-526893
ABSTRACT
AIM:
To study the effect of oridonin on the phagocytosis of apoptotic U937 cells by macrophage-like cells.METHODS:
DNA agarose gel electrophoresis, Giemsa staining, Hoechst 33258 staining and photomicroscopical observation were used.RESULTS:
UV irradiation (2.4 J/cm~2, 4 min) induced U937 cell apoptosis. Marked DNA fragmentation in agarose gel electrophoresis was observed. Oridonin augmented phagocytosis of apoptotic U937 cells by U937 cell-derived macrophages in a time- and dose-dependent manner. However, less effect on synthetic fluoresbrite micropheres was observed. The oridonin-augmented phagocytosis was attenuated by anti-human TNF? or anti-human IL-1? antiserum. In addition, the similar effect of phagocytosis was observed in oridonin-treated human monocyte-derived macrophages at 4 day maturation.CONCLUSION:
Oridonin enhances phagocytosis of apoptotic U937 cells by macrophage-like cells. The releases of TNF? and IL-1? are involved in this mechanism.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1986
Type:
Article
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