Effects of insulin on monocytic cytokines production and expression of HLA-DR in human septics / 中国普通外科杂志

ABSTRACT

Objective To observe the in vitro cytokines production and expression of human leukocyte antigen DR (HLA-DR) in monocytes in human septics. Methods Monocytes were isolated from 10 septic patients to observe the secretion function of TNF-?, IL-6 and IL-10 and HLA-DR expression in monocytes. Monocytes were isolated and incubated with different concentration of insulingroup A ( no insulin),group B (10 ?U/ml) ,group C (100 ?U/ml) ,group D (1000 ?U/ml) and group E (5000 ?U/ ml) , for 24 hours at 37 ℃. The levels of TNF-?, IL-6 and IL-10 in the supernatants were tested by ELISA. The antigen presenting function represented by HLA-DR expression was detected by flow cytometry analysis. Results With lower insulin concentration of culture media,secretion of TNF-? and IL-6 did not change in monocytes after excitation by LPS. When the insulin concentration elevated to 1000 ?U/ml, monocyte excretion of TNF-a and IL-6 was on significant depression (group D vs. group A,P