Determination of Vitamin B12 in Compound Vitamin Capsules by HPLC / 中国药房
China Pharmacy
;
(12)1991.
Article
in Chinese
| WPRIM
| ID: wpr-527361
ABSTRACT
OBJECTIVE:
To establish a HPLC method for determination of Vitamin B12 in compound vitamin capsules.METHODS:
Compound vitamin capsules were determined by HPLC on a ODS column at room temperature.The mobile phase consisted of methyl alcohol and 0.05mol/L KH2PO4 buffer(22∶78,pH=6.4). The flow rate was 1.0ml/min,and the detection wave length was set at 360nm.The sample size was 20?l.RESULTS:
The sample size of vitamin B12 presented a good linear relationship with peak area in the range of 10.20?g~204.00?g(r=0.9 999).The average recovery was 99.7%(RSD=1.18%).CONCLUSIONS:
The present method is simple,convenient,and accurate for determination of vitamin B12 in compou_nd vitamin capsules,and it also has the advantage of preventing the interference from vitamin B6 and folic acid.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
1991
Type:
Article
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