Functional activation of integrin?v?3 in bone marrow micrometastasis of breast cancer / 中国普通外科杂志

ABSTRACT

Objective To investigate the function of integrin ?v?3 receptor in bone marrow micrometastasis in breast cancer. Methods Flow cytometry was used to examine the expression of integrin ?v?3 receptor in breast cancer cell lines MCF7, MDA-MB-231 and MDA-MB-435s. Functional activation of integrin ?v?3 was assayed by cell adhesion, cell migration in Boyden Chamber and by confocal microscopy. The role of LM609 in bone marrow micrometastasis in athymic mice was detected by quantitative real-time RT-PCR. Results Integrin ?v?3 expression in cellular membrane was positive in MDA-MB-231 and MDA-MB-435s cell lines and negative in MCF-7 cell line. Cell adhesion and migration was attenuated significantly by LM609 in MDA-MB-231 and MDA-MB435s cell lines. Treated by LM609, the number of tumor cells significantly decreased in high integrin ?v?3 expression tumor in nude mice model(P