The inhibitive effect of the carboplatin-Fe@C nanocage-loaded chitosan nanoparticles on the proliferation of human hepatoma cell line HepG2 / 中国普通外科杂志

ABSTRACT

Objective To prepare the carboplatin-Fe@C nanocage-loaded chitosan nanoparticles(C-Fe@CN-CN),and observe the inhibitive effect on the proliferation of human hepatoma cell line HepG2 in vitro.Methods The C-Fe@CN-CN were prepared by the reverse microemulsion method and the character and drug release in vitro were observed.The inhibitive effect on the proliferation of the HepG2 cell was measured by MTT colorimetry,IC_(50) was calculated and the growth curve of HepG2 cell was drawn.Results C-Fe@CN-CN were in good spherical shape.The average size was 207nm?21nm with narrow distribution.The drug content was 11.40?1.31%.After a fast release during the first day,a more gradual drug release was sustained for another 4 days.C-Fe@CN-CN could apparently inhibit the proliferation of HepG2 cell inthe dose-dependent and time-dependent manner.The inhibitive effect of C-Fe@CN-CN at 24 hours was lower than that of original carboplatin,and was equal to original carboplatin at 48 hours and 72 hours.IC_(50) of C-Fe@CN-CN at 24h,48h and 72h was 135?g/ml,18.84?g/ml and 6.09?g/ml respectively.Bland nanoparticles had no cytotoxicity on HepG2 cell.Conclusions C-Fe@CN-CN possess strong magnetic responsivity,drug controlled(releasing) performance and the potential abilities of long circulation and permeation in tumor tissue.C-Fe@CN-CN can effectively inhibit the proliferation of HepG2 cell,and the blank nanoparticles express favourable biocompatibility in vitro.