Determination of Pazufloxacin Mesilate Concentration in Human Plasma and Urine by RP-HPLC / 中国药房
China Pharmacy
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-527835
ABSTRACT
OBJECTIVE:
To establish a RP-HPLC analytical method for the determination of pazufloxacin mesilate in human plasma and urine.METHODS:
The plasma proteins were precipitated with methanol and the supernatant liquid ob-tained from the serum centrifugate was subjected to chromatographic analysis.The supernatant liquid obtained from the diluted urine centrifugate was subjected to sample introduction.The analytical column was Diamonsil C 18 ,the mobile phase consisted of acetonitrile-0.05mol/L potassium dihydrogen phosphate(containing1%tetrabutylammonium bromide)(8∶92,V/V)with a flow rate at1.4ml/min,excitation wavelength at320nm and emission wavelength at400nm.RESULTS:
The linear range of pazufloxacin mesilate in both plasma and urine was31.25~10000ng/ml(r=0.9999).The relative recoveries of pazu-floxacin mesilate in human plasma and urine were97.77%~99.87%and98.31%~100.82%,respectively with RSD less than1.0%~3.0%.CONCLUSION:
This method is accurate,reliable and simple and it is suitable for the pharmacokinetic study and routine monitoring of blood concentration of pazufloxacin mesilate.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2001
Type:
Article
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