The signal transducers involved in the regulation of calcium sensitivity of vascular smooth muscle in hemorrhagic shock / 中国病理生理杂志

ABSTRACT

AIM: To observe the regulatory effects of Rho-kinase,PKC and PKG on calcium sensitivity of vascular smooth muscle in hemorrhagic shock in rats.METHODS: The superior mesenteric artery(SMA) from hemorrhagic shock model of rat was adopted to assay the calcium sensitivity via observing the contraction initiated by Ca~(2+) under depolarizing conditions(120 mmol/L K~+) with isolated organ perfusion system.Rho-kinase agonist Ang-Ⅱ and inhibitor fasudil,PKC agonist PMA and inhibitor staurosporine,PKG agonist 8Br-cGMP and inhibitor KT-5823 were used as tool agents to study the regulatory effect of Rho-kinase,PKC and PKG on the calcium sensitivity of SMA following shock.RESULTS: Ang-Ⅱ,PMA and KT-5823 improved the calcium sensitivity of SMA and made the cumulative dose-response curve of SMA to Ca~(2+) shift to the left,their Emax of Ca~(2+)(at 3?10~(-2) mol/L) was 0.630 g/mg,0.595 g/mg and 0.624 g/mg,respectively,which were all higher than that in shock control(0.377 g/mg)(P