Construction and identification of mouse telomerase reverse transcriptase eukaryotic expression vector / 中国病理生理杂志

ABSTRACT

AIM: To clone and express mouse telomerase reverse transcriptase(mTERT) cDNA and obtain eukaryotic expression vector.METHODS: The cDNA of mTERT was amplified by RT-PCR and PCR.After purification,the gene fragment was cloned into a vector PUC-19.The sequence of inserted mTERT gene fragment was also detected.RESULTS: The recombinant plasmid PUC-19-mTERT was constructed.Positive clones were screened and identified by PCR and digestion with restriction enzyme.The size of gene fragment was(3 369) bp and in accordance with the expected one.CONCLUSION: The mTERT cDNA was obtained and the recombinant eukaryotic expression vector was successfully constructed.The study lays foundation for DCs vaccine modified by mTERT gene for the treatment of tumor.