Effects of Electroacupuncture at Jiaji(EX-B_2)on Phosphorylated ERK and NK-1 Signal Conduction Pathway in Dorsal Horn in Complete Freund's Adjuvant Arthritis Rats / 中医杂志

ABSTRACT

Objective:To observe effects of electroacupuncture at Jiaji(EX-B_2)on phosphorylated extracellular signal regulated kinase(ERK)and neurokinin-1(NK-1)in dorsal horn of the complete Freund's adjuvant arthritis rat,so as to study possible mechanism of electroacupuncture analgesia from the point of view of signal conduction.Methods:Eighty Wistar adult male rats were randomly divided into normal control group,simple electroacupuncture(EA)group,model 30 min group,model 24h group,model 48 h group,EA 30min group,EA 24h group,and EA 48h group,10 rats in each group.Inflammatory pain rat model was established by injection of complete Freund's adjuvant into left hindpaw.EA was given at bilateral L_3～L_5 Jiaji(EX-B_2).The pain threshold was determined and phosphorylated ERK and NK-1 expressions in the spinal cord were detected by immunohistochemical technique.Results:The pain threshold significantly decreased(all P