The construction of MUCI-VNTR DNA vaccine for pancreatic cancer / 中国普通外科杂志
Chinese Journal of General Surgery
;
(12)1994.
Article
in Chinese
| WPRIM
| ID: wpr-527951
ABSTRACT
Objective To construct MUC1-VNTR DNA vaccine pancreatic cancer. Methods The recombinant gene of VNTR was synthesized and cloned into MCS in the pcDNA3. 1/Myc-his ( + ) A vector. pcDNA3. 1-VNTR/Myc-his( + ) A was injected twice into C57BL/6( H-2b)female mice (V group, n = 15). Mice inoculated with either the empty plasmid vector ( D group, n = 15 ) or 0. 9% NaCl ( NS group, n, = 15) were used as control. Two weeks later, both humoral and cellular immunity of the mice were studied. Results The recombinant plasmid pcDNA3. 1 -VNTR/Myc-his ( + ) A encoded the whole exact translation frame region of the pcDNA3. 1/Myc-his ( + ) A vector and the recombinant gene of human VNTR. The transfected COS7 cells expressed transgene products at 48 hours after transfection. Intramuscular delivery of the recombinant plasmid into C57BL/6 mice resulted in more efficient induction of CTL lysis specific against VNTR polypeptide than the D group and the NS group (P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of General Surgery
Year:
1994
Type:
Article
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