A study on the influential factors of G6PD/6PGD specific value assay in the heterozygotes of G6PD gene variants in female patients / 国际检验医学杂志
International Journal of Laboratory Medicine
;
(12)2006.
Article
in Chinese
| WPRIM
| ID: wpr-528053
ABSTRACT
Objective To improve the detection of G6PD heterozygotes in female patients by the optimum experimental factors of G6PD/6PGD specific value assay.Methods (1)Identifying the mutations of G6PD gene with the use of amplified refractory mutation system (ARMS).(2)Measuring the G6PD/6PGD specific value.Results According to the data analysed by statistics and ROC curve, the optimum experimental factors included that the incubation temperature was 37℃,the substrate concentrations were 0.78 mmol/L G6PNa2 and 0.195mmol/L NADP+, the reaction time was 10min.Conclusion The optimum experimental factors of G6PD/6PGD specific value assay may be used to improve the detection rate of G6PD heterozygotes in female patients.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
International Journal of Laboratory Medicine
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS