Influence of diethylstilbestrol on expressions of proliferating cell nuclear antigen and ACTIN in gubernaculum testis of fetal male mice / 中国医师杂志

ABSTRACT

Objective To study the effects of prenatal exposure to diethylstilbestrol(DES) on expressions of ACTIN and proliferating cell nuclear antigen(PCNA) in the development of gubernaculum testis of fetal male mice.Methods Pregnant mice were randomly divided into 6 groups and injected with DES subcutaneously from the 9th gestational day to the 17th day at dosages of 0,25,50,100,200 ?g?kg~(-1)?d~(-1) dissolved in 0.2ml dimethyl sulfoxide(DMSO).The mice in the control group were given normal saline alone.The mice were sacrificed and the fetuses were quickly removed for fixation on the 17th day.Histological changes were observed with light microscope by sliced serially in coronal plane.The expressions of ACTIN and PCNA in gubernaculum testis were detected by immunohistochemical methods.Results In experimental groups,the gubernaculum testis of mice was hypoplasia.The expressions of ACTIN and PCNA in gubernaculum testis were lower in the DES treated groups than those in the control group with obvious dose-effect correlation(P