Determination of Chlorogenic Acid and Baicalin in Xiaoer Qingrening Granula by HPLC / 中国药房
China Pharmacy
;
(12)2005.
Article
in Chinese
| WPRIM
| ID: wpr-528376
ABSTRACT
OBJECTIVE:
To establish a HPLC method for the determination of chlorogenic acid and baicalin in xiaoer qingrening granula.METHODS:
The determination was performed on Symmetry Shield RP C 18 ,the mobile phase of chlorogenic acid consisted of acetonitrile-0.4%phosphoric acid soluton(13∶87)and that of baicalin consisted of methanol-water-glacial acetic acid(50∶50∶1),the detection wavelengths of chlorogenic acid and baicalin were327nm and274nm,respectively,the flow rate was1.0ml/min,sample size was20?l and column temperature was25℃.RESULTS:
The respective linear sample size range for chlorogenic acid and baicalin was0.1040?g~1.040?g(r=0.9998)and0.6240?g~6.240?g(r=0.9997),with average recoveries respectively at96.4%(RSD=1.4%)and98.0%(RSD=1.1%).CONCLUSIONS:
The method is simple,accurate,reproducible and specific,and it can be used for the quality control of xiaoer qingrening granula.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS